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GenoFrag - version 2.1 (2009)
D. Lavenier
copyright IRISA/CNRS - INRA

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The distribution is composed of the following files

  - genofrag.py: the last python version of genofrag
  - primers: a file containing 500 primers
  - readme: a short documentation


To run GenoFrag:

   python genofrag.py


To test GenoFrag:

   1 - enter "primers" inside the window  "Primer file"
   2 - push the RUN button
   3 - check the result: a solution is found

   4 - dicrease the overlap between primers (set min to 950 and max to 1000)
   5 - check the result: no solution found

   6 - increase the overlap between primers (set max to 1050)
   7 - check the result: a solution is found

   8 - save the results: push the SAVE button
   9 - check the results have been written into the genofrag.res text file.


Please cite

   N. Ben Zakour, M. Gautier, R. Andonov, D. Lavenier, M-F. Cochet, P. Veber, 
   A. Sorokin, Y. Le Loir,  GenoFrag: software to design primers optimized for 
   whole genome scanning by long-range PCR amplification, 
   Nucleic Acids Research,32(1), 2004.


Contact

   Dominique LAVENIER -- lavenier@irisa.fr 

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